Nucleosome, Recombinant Human, H3K4me1 dNuc, Biotinylated – 50 µg

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3*, and H4) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains monomethyl-lysine at position 4. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group. *Histone H3.2 has a Cys to Ala substitution at position 110.