SP1 CUTANA™ CUT&RUN Antibody
8 in stock
Powered by BiozThis antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. SP1 is the original member of the Sp1-like family of zinc-finger transcription factors. It binds GC-rich motifs with high affinity [1] and is involved in expressing genes related to cell growth, cell-cycle progression, survival, and tumorigenesis [2]. SP1 antibody produces CUT&RUN peaks above background primarily in intronic and promoter regions (Figure 1) that overlap with known SP1 DNA-binding motifs (Figure 2).
Trademarks and copyrights
CUT&RUN was performed on 500k HeLa cells with 0.5 µg of either SP1 or IgG negative control (EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 5.0 million reads (IgG) and 5.3 million reads (SP1). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: SP1 peaks in CUT&RUN
Figure 2: SP1 transcription factor binding motif analysis in CUT&RUN
Figure 3: SP1 CUT&RUN representative browser tracks
Figure 4: Immunohistochemistry data
Figure 5: Immunoprecipitation data
Figure 6: Western blot data
- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, IHC, IP, WB
- Reactivity: Human, Mouse
- Format: Antigen affinity-purified
- Target Size: 81 kDa
- CUT&RUN: 0.5 µg per reaction
- Immunohistochemistry (IHC): 1:2,000 – 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Immunoprecipitation (IP): 2 – 10 µg/mg lysate
- Western Blot (WB): 1:2,000 – 1:10,000
- UniProt ID: P08047
- Gene Name: SP1
- Protein Name: Transcription factor SP1
- Target Size: 81 kDa
- Alternate Names: specificity protein 1, transcription factor SP1, TSFP1
