TP53/p53 CUTANA™ CUT&RUN Antibody
11 in stock
Powered by BiozThis antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. TP53/p53 acts as a tumor suppressor by promoting growth arrest or apoptosis and is mutated in over 50% of cancers. It forms a complex with NF-Y, which binds to CCAAT box-containing promoters [1]. TP53/p53 antibody produces CUT&RUN peaks above background primarily in promoter regions (Figure 1) that are enriched with known TP53/p53 DNA-binding motifs (Figure 2).
Trademarks and copyrights
Background References:
[1] Benatti et al. Nucleic Acids Res (2008). PMID: 18187512
CUT&RUN was performed on 500k HeLa cells with 0.5 µg of either TP53/p53 or IgG negative control (EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 5.1 million reads (IgG) and 5.9 million reads (TP53). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: TP53/p53 peaks in CUT&RUN.
Figure 2: TP53/p53 transcription factor binding motif analysis in CUT&RUN.
Figure 3: TP53/p53 CUT&RUN representative browser tracks.
Figure 4: Immunoprecipitation data.
Figure 5: Western blot data.
- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, IP, WB
- Reactivity: Human
- Format: Antigen affinity-purified
- Target Size: 43 kDa
Between amino acids 325 and 375
Formulation
Antigen affinity-purified antibody in Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide
- CUT&RUN: 0.5 µg per reaction
- Immunoprecipitation: 2-10 μg/mg lysate
- Western Blot: 1:2,000 – 1:10,000
- UniProt ID: P04637
- Gene Name: TP53
- Protein Name: Cellular tumor antigen p53
- Target Size: 43 kDa
- Alternate Names: antigen NY-CO-13, BCC7, LFS1, mutant tumor protein p53, P53, p53 tumor suppressor, TRP53
