Nucleosome, Recombinant Human, H3R2me2s dNuc, Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2BO60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pair of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains symmetrically dimethyl-arginine at position 2. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains 5’ biotin-TEG group.
*H3R2me2s has a Cys to Ala substitution at position 110.