Nucleosome, Recombinant Human, H3K9cr dNuc, Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains crotonyl-lysine at position 9. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom, has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group.
* H3K9cr has a Cys to Ala substitution at position 110.