Nucleosome, Recombinant Human, H3K27me1 dNuc, Biotinylated

Recombinant mononucleosomes (H3K27me1) consist of 147 base pairs of DNA wrapped around an octamer core of histone
proteins (two each of H2A, H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601
sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly.
H3K27me1 dNuc contains monomethyl-lysine at position 27 on histone H3.2 and has a Cys to Ala substitution at position
110. The DNA in this nucleosome contains a 5’biotin-TEG group.