Mononucleosomes, Recombinant, Symmetrically Methylated 199×601 DNA, Biotinylated

Recombinant mononucleosomes consist of 199 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 601 sequence, identified by Lowary and Widom [1], is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly. The 601 sequence is flanked by a symmetrically methylated (bolded/blue) 26 bp sequence (underlined) as shown in the DNA sequence below. The DNA in this nucleosome contains a 5’ biotin-TEG group.