Mononucleosomes, Recombinant Human, Desthiobiotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1, and H4 Accession numbers: H2A-P04908; H2BO60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ desthiobiotin-TEG group. Desthiobiotin has a weaker affinity (K_d = 10-11) for avidin-family proteins than does biotin (K_d= 10-15).