Mononucleosomes, Recombinant, 199×601 DNA, Non-Biotinylated

Mononucleosomes assembled from recombinant histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P06897; H2B-P02281; H3- Q92133; H4-P62799) wrapped by 199 base pairs of 601 positioning sequence DNA. The 601 sequence, identified by Lowary and Widom [1], is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly. The 601 sequence is flanked by a 26 bp sequence as underlined in application notes.