Mononucleosomes (H4 ΔN15), Human Recombinant Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4 (ΔN15). Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) with the amino acid sequence of H4 beginning with lysine 16 (amino acids 1-15 are deleted), wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for
nucleosome assembly and contains a 5’ biotin-TEG group.