Mononucleosomes (H3.1 ΔN32), Human Recombinant

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1 and H4. Accession numbers: H2A-P04908; H2BO60814; H3.1-P68431; H4-P62805), with the amino acid sequence of H3 beginning with glycine 33 (amino acids 1-32 are deleted), wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly.