Mononucleosome (H3.1 ΔN2), Recombinant Human, Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1ΔN2, and H4 Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) with the amino acid sequence of H3 beginning with threonine 3, wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.