Mononucleosomes (H2AZ.2), Human Recombinant Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2AZ.2, H2B, H3 and H4. Accession numbers: H2AZ.2-Q71UI9; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA with a 5’ biotin-TEG group. The nucleosome is the basic subunit of chromatin. H2AZ.1 and H2AZ.2 are variants of histone H2A and implicated in diverse cellular functions ranging from transcriptional regulation, chromosome transmission and DNA damage repair. H2AZ has a dedicated deposition machinery (the SWR-C ATPase). The DNA in this nucleosome contains a 5’ biotin-TEG group.