Mononucleosomes (H2AX), Human Recombinant Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2AX, H2B, H3, and H4) wrapped by 147 base pairs of 601 positioning sequence DNA with a 5’ biotin-TEG group. The nucleosome is the basic subunit of chromatin. H2AX is a variant of histone H2A that is phosphorylated on serine 139 by the ATM and ATR kinases in response to DNA double strand breakage. Phosphorylation of H2AX serves to recruit a number of DNA damage repair proteins, such as BRCA1 and NBS1.