JUN/c-Jun CUTANA™ CUT&RUN Antibody
33 in stock
Powered by BiozThis antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. JUN/c-Jun heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex and binds to the AP-1 consensus motif [1]. JUN/c-Jun antibody produces CUT&RUN peaks above background primarily in intronic, intergenic, and promoter regions (Figure 1) that overlap with known JUN/c-Jun DNA-binding motifs (Figure 2).
Trademarks and copyrights
Background References:
[1] van Dam & Castellazzi Oncogene (2001). PMID: 11402340
CUT&RUN was performed on 500k HeLa cells with 0.5 µg of either JUN/c-Jun or IgG negative control (EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 5.1 million reads (IgG), 12.1 million reads (JUN/c-Jun), and 6.2 million reads (H3K4me3). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: JUN/c-Jun peaks in CUT&RUN
Figure 2: JUN/c-Jun transcription factor binding motif analysis in CUT&RUN
Figure 3: JUN/c-Jun CUT&RUN representative browser tracks
Figure 4: Immunohistochemistry data
Figure 5: Immunoprecipitation data
Figure 6: Western blot data
- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, IHC, IP, WB
- Reactivity: Human, Mouse
- Format: Antigen affinity-purified
- Target Size: 35 kDa
- CUT&RUN: 0.5 µg per reaction
- Immunohistochemistry (IHC): 1:100 – 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Immunoprecipitation (IP): 2– 5 µg/mg lysate
- Western Blot (WB): 1:2,000 – 1:10,000
- UniProt ID: P05412
- Gene Name: JUN
- Protein Name: Transcription factor Jun
- Target Size: 35 kDa
- Alternate Names: transcription factor Jun, activator protein 1, AP1, proto-oncogene c-Jun, transcription factor AP-1 subunit Jun, v-jun avian sarcoma virus 17 oncogene homolog, p39, AP-1, c-Jun, enhancer-binding protein AP1, Jun activation domain binding protein, jun oncogene
