Heterotypic [H3K4me3 • H3K14ac] Recombinant Nucleosome, Biotinylated

Heterotypic [H3K4me3 • H3K14ac] Recombinant Nucleosome, Biotinylated is a fully defined semi-synthetic nucleosome
containing combinatorial PTMs in trans whereby one histone H3 is trimethylated at lysine four and the other is acetylated at
lysine 14. [H3K4me3 • H3K14ac] nucleosome consists of 147 base pairs of 601 sequence DNA [2] wrapped around an
octamer of core histone proteins (two each of H2A, H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of
chromatin. The DNA contains a 5’ biotin-TEG group, and histone H3.2 has a Cys to Ala substitution at position 110. The
NURF chromatin remodeling complex subunit BPTF engages heterotypic target PTMs in cis [H3 • H3K4me3,K14ac] as
opposed to trans [H3K4me3 • H3K14ac] through tandem PHD finger and bromodomain (PHD-BD) reader interactions [3].