Heterotypic [H3 • H3K4me3] Recombinant Nucleosome, Biotinylated

Heterotypic [H3 • H3K4me3] Recombinant Nucleosome, Biotinylated is a fully defined semi-synthetic nucleosome containing
one unmodified histone H3 and one histone H3 with trimethylated lysine at position four. [H3 • H3K4me3] nucleosome
consists of 147 base pairs of 601 sequence DNA [2] wrapped around an octamer of core histone proteins (two each of H2A,
H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of chromatin. The DNA contains a 5’ biotin-TEG group, and
histone H3.2 has a Cys to Ala substitution at position 110. Heterotypic [H3 • H3K4me3] nucleosomes fail to recruit known
H3K4me3 binding proteins, including members of the TFIID, SETD1, and SIN3A/B complexes; chromatin remodelers NURF,
CHD1, and CHD8; and PHD finger protein PHF2, suggesting that heterotypic nucleosomes may regulate transcription by
affecting the recruitment of various chromatin regulatory complexes [3].