H3.3G34R Mononucleosome, Biotinylated

H3.3 is a histone variant found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of R for G at position 34 of H3.3 interferes with SETD2-mediated H3K36 methylation. This mutation has been associated with bone cancers and astrocytomas[1]. H3.3G34R Mononucleosomes consist of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.3G34R and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [2], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA in this nucleosome contains a 5’ biotin-TEG group.