EpiDyne Remodeling Assay Substrate DNA ST601-GATC1

EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1 is a 217 base-pair double-stranded DNA fragment. The sequence includes an upstream 6 bp linker, the 145 bp ST601 nucleosome positioning sequence, identified by Lowary and Widom [1], and a downstream 66 bp linker (hereby named 6-N-66 DNA). The DNA also includes a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling. This positive control has a DpnII restriction site within the 601 sequence. When paired with the negative control (EpiCypher 18-4100), these controls illustrate the migration range for the Restriction Enzyme Assay. For more information, see the EpiDyne Nucleosome Remodeling Assay Tech Note: epicypher.com/resources/technical-notes/