EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated

EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated consists of 263 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 263 base pair DNA sequence includes an upstream 50 bp linker, the 147 bp ST601 nucleosome positioning sequence, identified by Lowary and Widom [1], and a downstream 66 bp linker (hereby named 50-N-66 nucleosome). One copy of the ‘GATC’ DNA motif is embedded within the ST601 sequence and there is a 5’ biotin-