ELF1 CUTANA™ CUT&RUN Antibody
8 in stock
Powered by BiozThis antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. ELF1 is a member of the ETS (e26 transformation specific sequence) family of transcription factor proteins. ELF1 antibody produces CUT&RUN peaks above background primarily in intronic and promoter regions (Figure 1) that overlap with known ELF1 DNA-binding motifs (Figure 2). ELF1 is expressed in B and T cells and has been shown to be involved with the regulation of several T- and B-cell specific genes [1].
Trademarks and copyrights
Background References:
[1] Oettgen et al. Mol Cell Biol (1996). PMID: 8756667
CUT&RUN was performed on 500k HeLa cells with 0.5 µg of either ELF1 or IgG negative control (EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 5.0 million reads (IgG) and 6.0 million reads (ELF1). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: ELF1 peaks in CUT&RUN.
Figure 2: ELF1 transcription factor binding motif analysis in CUT&RUN
Figure 3: ELF1 CUT&RUN representative browser tracks
Figure 4: Immunohistochemistry data
- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, IHC
- Reactivity: Human
- Format: Antigen affinity-purified
- Target Size: 67 kDa
Immunogen
Between amino acids 569 and 619
Antigen affinity-purified antibody in Tris-buffered saline, 0.1% BSA, 0.9% sodium azide
- CUT&RUN: 0.5 µg per reaction
- Immunohistochemistry (IHC): 1:100 – 1:500
- UniProt ID: P32519
- Gene Name: E74 like ETS transcription factor 1
- Protein Name: ETS-related transcription factor Elf-1
- Alternate Names: ETS-like factor 1, EFTUD1, RIA1
- Target Size: 67 kDa
