SKU: 21-1027-S or 21-1027-L
CUTANA™ Protease Inhibitor Tablets
Price range: $235.00 through $505.00
Powered by BiozThis EDTA-free cocktail inhibits various serine and cysteine proteases, resulting in stabilization of target proteins isolated from animal tissues, plants, and yeast for in situ assays like ChIC/CUT&RUN (EpiCypher 14-1048) or CUT&Tag (EpiCypher 14-1102/14-1103). Absence of EDTA and other chelating agents is essential for the controlled activation of pAG-MNase (EpiCypher 15-1016/15-1116) and pAG-Tn5 (EpiCypher 15-1017/15-1117), but leaves metallo- and aspartic proteases inhibited. This product is also used in the preparation of CUTANA™ Nuclei Extraction Buffer (EpiCypher 21-1026).
Storage
Tablets stored in their original container are stable at 4ºC until the date of expiration on the label.
Formulation
Proprietary mixture of protease inhibitors; EDTA free.
Instructions for Use
Reconstitute each tablet in 420 µL (15-1027-S) or 2 mL (15-1027-L) molecular biology-grade water to prepare a 25X stock. Leftover 25X protease inhibitor can be stored for 12 weeks at -20ºC.
Reactions/Tablet (approx.)
| 15-1027-S | 15-1027-L | |
| CUT&RUN | 5 reactions | 27 reactions |
| CUT&Tag | 6 reactions | 30 reactions |
| Nuclei Extraction | 40 reactions | 204 reactions |
