CUTANA™ Anti-GST Tag Antibody
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Unlock the full potential of Reader CUT&RUN with our high-affinity CUTANA™ Anti-GST Tag Antibody, specifically developed to support chromatin profiling applications wherein a GST-tagged reader protein replaces the traditional primary antibody in directing the selective cleavage and release of target chromatin fragments by pAG-MNase [1]. For example, this antibody is used in CUTANA™ meCUT&RUN, a modified CUT&RUN workflow in which a GST-tagged MeCP2 methyl binding domain is used to enrich regions of chromatin with symmetrically methylated CpGs, enabling streamlined, high-resolution mapping of DNA methylation.
Engineered for superior performance in low-background, high-resolution CUT&RUN assays, this antibody offers exceptional sensitivity and specificity for detecting GST-fusion proteins bound to chromatin. Whether studying histone modifications, DNA methylation, or other epigenetic readers, this antibody is the ideal tool for enabling precise, reproducible mapping using GST-tagged fusion constructs.
Background References:
[1] Marunde et al. eLife (2024). PMID: 38319148
Figure 1: meCUT&RUN DNA fragment size distribution analysis
Figure 2: Gene browser tracks
Figure 4: meCUT&RUN methods
meCUT&RUN was performed starting with 500k K562 cells with either 2.5 μL of 20X GST-MeCP2 (EpiCypher 15-2002) added as the primary binding reagent or 0.5 μg of a secondary antibody-only control (anti-GST antibody) to determine background cleavage. Library preparation was performed using 5 ng of meCUT&RUN-enriched DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001). Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 7.7 million reads (anti-GST) and 8.4 million reads (GST-MeCP2). Data were aligned to the hg38 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
ITEM | CAT. NO. |
---|---|
GST-MeCP2 for meCUT&RUN | 15-2002 |
CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
Western Blot (WB):