Acidic Patch Mutant H2AE61A Recombinant Nucleosome with Linker DNA, Biotinylated

H2AE61A Recombinant Nucleosome with Linker DNA consists of 199 base pairs of DNA wrapped around an octamer core of
histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 5’
biotin-TEG DNA consists of a core 147 bp 601 nucleosome assembly sequence [4] flanked by 26 bp linker sequences as
underlined below. Histone H2A contains a glutamate-to-alanine (E-to-A) substitution within the acidic patch at position 61
(H2AE61A). H2AE61 resides in the alpha2 helix, forming a key salt bridge with H4K16, and mediates chromatin binding with
factors such as LANA, RCC1, IL-33, Sir3, and HMGN2 [1]. H2AE61A disrupts chromatin remodeling by SMARCA5/SNF2h, an
ATPase subunit of the ISWI family of chromatin remodeling complexes [2,3].