meCUT&RUN: DNA Methylation Landing Page
New CUTANA™
meCUT&RUN Assays for DNA Methylation Sequencing
An Affordable Solution for Genome-Wide DNA Methylation Sequencing
DNA methylation researchers face a tough choice: expensive whole-genome bisulfite sequencing (WGBS), or limited insights from arrays, reduced representation bisulfite sequencing (RRBS), and targeted panels. Bisulfite conversion also damages DNA, resulting in potential biases that can complicate data analysis.
CUTANA™ meCUT&RUN solves this challenge by selectively enriching methylated DNA, delivering high-quality, genome-wide insights at a fraction of the sequencing required for WGBS.
Why Choose meCUT&RUN:
- Capture 80% of methylated CpGs
- 20-fold reduced sequencing costs
- Robust down to 10,000 cells
- Avoids bisulfite conversion, minimizing bias
Limited-Time Discounts Available: Request a Quote!
We have everything you need to get started with CUTANA™ meCUT&RUN assays!
- CUTANA™ meCUT&RUN Kit: Everything you need to enrich methylated DNA via a modified CUT&RUN workflow.
- If Kits aren’t for you, we also offer a collection of individual meCUT&RUN reagents:
- GST-MeCP2 for meCUT&RUN: Enriches methylated DNA in meCUT&RUN
- Anti-GST Tag Antibody: Required for meCUT&RUN targeted cleavage
- Fragmented Controls for DNA Methylation Sequencing: Important controls for base-pair resolution DNA methylation sequencing
CUTANA™ meCUT&RUN: An Improved DNA Methylation Sequencing Approach
Enter CUTANA™ meCUT&RUN: Genome-wide DNA methylation insights for a fraction of the cost!
meCUT&RUN Workflow Enriches Methylated DNA for Streamlined 5-Methylcytosine Sequencing
Many 5-methylcytosine mapping strategies, like whole-genome bisulfite sequencing (WGBS), require >800 M reads, intensive bioinformatics processing, and high overall costs, making them impractical for many research labs. However, targeted strategies also raise concerns. Reduced representation bisulfite sequencing (RRBS), microarrays, and hybridization panels only cover a small percentage of CpG sites and are often biased for CpG islands. Furthermore, the use of bisulfite conversion introduces potential bias and damages DNA, resulting in high input requirements.
meCUT&RUN Delivers Robust DNA Methylation Profiles Without the Whole-Genome Sequencing Price Tag
We paired meCUT&RUN with Enzymatic Methyl-seq (EM-seq) to enable base-pair resolution comparisons with RRBS and Whole-Genome EM-seq (Figure 2). The results: CUTANA™ meCUT&RUN generates DNA methylation profiles similar to whole genome EM-seq at greatly reduced sequencing depth, while also providing improved 5-methylcytosine coverage compared to RRBS.
At 20-30 M unique reads, meCUT&RUN detects 80% of methylated CpGs, providing improved coverage vs. other targeted approaches, while also reducing sequencing costs.
meCUT&RUN is a robust approach for capturing genome-wide DNA methylation, even at low sequencing depths. Figure 3 shows the number of methylated CpGs (5mCs) detected by meCUT&RUN at varying sequencing depths, normalized to the total number of 5mCs captured by Whole-Genome EM-seq.
The CUTANA™ meCUT&RUN Advantage in DNA Methylation Profiling: Sequence MORE of the Methylome, Even from Low Cell Numbers
If you need an efficient, cost-effective, and genome-wide method for DNA methylation analysis, meCUT&RUN is for you!
meCUT&RUN assays are useful for a variety of biomedical research areas, including:
💊 Drug and biomarker discovery teams
🧫 Studies with human cell lines and tissues
🐁 Mouse models of cancer, metabolic disease, and development
🧠 Neuroscience research
