Histone H4K12ac Antibody, SNAP-ChIP® Certified
7 in stock
Powered by BiozThis antibody meets EpiCypher’s “SNAP-ChIP® Certified” criteria for specificity and efficient target enrichment in a ChIP experiment (<20% cross-reactivity across the panel, >5% recovery of target input). Histone H4 is one of the four proteins that are present in the nucleosome, the basic repeating subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). This antibody reacts to H4K12ac, however its binding to the target is negatively impacted by nearby acetylations (e.g. tetraAc-H4). No cross reactivity with other lysine acylations in the EpiCypher SNAP-ChIP K-AcylStat panel, is detected.
Figure 1: SNAP-ChIP qPCR Data
Figure 2: Luminex Data
Histone H4K12ac antibody was assessed using a Luminex® based approach employing dCypher™ Nucleosome K-AcylStat Panel (EpiCypher Catalog No. 16-9003). The panel comprises biotinylated designer nucleosomes (X-Axis) individually coupled to uniquely identifiable Luminex Magplex® beads. Antibody binding to nucleosomes was tested in multiplex (24-plex) at a 1:1000 dilution, and detected with second layer anti-IgG*PE. Data was generated using a Luminex FlexMAP3D®. Data normalized to relevant on-target (H4K12ac; set to 100) is shown. tetraAc-H3 is K4,K9,K14,K18ac; tetraAc-H4 is K5,K8,K12,K16ac; tetraAc-H2A is K5,K8,K13,K15ac
Figure 3: Western Blot Data
Western blot of acid extracts from HeLa cells treated with sodium butyrate ( 1 ), acid extracts from untreated HeLa cells ( 2 ) using EpiCypher Catalog No. 13-0037 at 0.5 µg/mL.
Figure 4: Immunocytochemistry Data
Immunocytochemistry of HeLa cells treated with sodium butyrate, using H4K12ac antibody, EpiCypher Catalog No. 13-0037 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
References
[1] Grzybowski et al (2015) Mol Cell 58:886
[2] Shah et al (2018) Mol Cell 72:162
- Type: Monoclonal
- Mol Wgt.: 11.4 kDa
- Format: Aff. Pur. IgG
- Host: Rabbit
- Appl.: ChIP, ChIP-Seq, WB, ICC, L
- Reactivity: H, M, WR
Immunogen
A synthetic peptide corresponding to histone H4 acetylated at lysine 12.
Formulation
Protein A affinity-purified recombinant monoclonal antibody (1 mg/mL) in PBS, with 0.09% sodium azide, 1% BSA, and 50% glycerol.
Storage and Stability
Stable for 1 years at -20°C from date of receipt.
- ChIP: 2 – 5 μg per 1×106 cells
- WB: 1 – 2 μg/mL
- ICC: 0.05 – 2 μg/mL
- L: 0.25 – 4 μg/mL