Nucleosome, Recombinant Human, H3K4,K27me3 dNuc, Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B,
H3 and H4; accession numbers: H2A-P20671; H2B-O60814; H3.2-Q71DI3; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains trimethylated-lysines at positions 4 and 27. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group.
* H3K4,K27me3 has a Cys to Ala substitution at position 110.