Symmetric dimethylation of arginine (sDMA) is a post-translational modification catalyzed by type II arginine methyltransferase enzymes and found on many proteins,including those involved in transcriptional regulation, mRNA splicing, DNA repair and nuclear transport. Sym10 recognizes SDMA present at GAR (glycine-arginine rich sequences).
Sym10 Antibody Immunogen:
KLH-conjugated peptide with the sequence KRGRGRGRG in which arg residues were symmetrically dimethylated.
Sym10 Antibody Formulation:
Rabbit serum with 30% glycerol and 0.035% sodium azide.
Sym10 Antibody Storage and Stability:
Stable for 2 years at -20°C from date of receipt.
Sym10 Antibody Application Notes:
Sym10 antibody is useful for Western blotting (1:500 - 1:2,000 dilution) to detect SDMA on a variety proteins.
Sym10 Antibody References:
Boisvert FM et al (2002) J Cell Biol 159: 957-969. Link Boisvert FM et al (2003) Mol Cell Proteom 2: 1319-1330. Link CÃÂ«te J et al (2005). J Biol Chem 280: 28476-28483. Link Deng X et al (2010). PNAS USA 107: 19114?19119. Link Jung GA et al (2011). Exp Mol Med 43:550-560. Link
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Western Blot Data: Western blot using Symmetric Dimethyl-arginine Antibody (SDMA) Sym10 (1:500 dilution) on mouse embryonic fibroblast (MEF) whole cell extract derived from cells with (+/+) or without (-/-) the PRMT5 gene. Sym10 detects multiple proteins in the ?PRMT5 +/+? cell extract lane, indicating proteins containing SDMA. PRMT5 is responsible for the majority of SDMA in mammals. The relative migration positions and molecular weights of protein size standards are indicated, as well as a Western blot recognizing the ?-actin protein, a loading control. (Click image to enlarge)