SNAP-ChIP® OncoStat™ Panel Primer Set

$175.00
SKU: 18-6201
Pack Size: 100 reactions
SNAP-ChIP® OncoStat™ Panel Primer Set: The SNAP-ChIP® OncoStat™ Panel Primer Set contains a universal forward primer pre-mixed with reverse primers that uniquely amplify DNA-barcoded nucleosomes in the SNAP-ChIP KMetStat Panel (Catalog No. 19-2001), consisting of H3.3 wild-type (WT), H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W, and H3.3K36M. These primers can be used in SNAP-ChIP experiments where quantitative PCR (qPCR) provides an assessment of antibody performance and technical variability. The primers are compatible with SYBR™ chemistry. For TaqMan® applications, the universal SNAP-ChIP Dual Label Hydrolysis Probe can be purchased separately (Catalog No. 18-6001). The volume is sufficient to perform 100 x 10 μL qPCR reactions (see SNAP-ChIP manual).

SNAP-ChIP® OncoStat™ Panel Primer Set Formulation: OncoStat universal forward primer pre-mixed with individual reverse primers at 5 μM (20X) individual primer concentration in Molecular Grade H2O.

SNAP-ChIP® OncoStat™ Panel Primer SetStorage and Stability: Stable for 2 years at -20°C from date of receipt.

SNAP-ChIP® OncoStat™ Panel Primer Set Application Notes: For use in SNAP-ChIP-qPCR. Compatible with the SNAP-ChIP KMetStat Panel (Catalog No. 19-2001) and the SNAP-ChIP Dual Label Hydrolysis Probe (Catalog No. 18-6001).

DNA Sequence:

SNAP-ChIP OncoStat Forward Primer 5’-CGTATCGCGCGCATAATA-3’
H3.3 WT Reverse Primer 5’-ATACGACGAGATAGTCGACG-3’
H3.3K4M Reverse Primer 5’-TCGCGCGTACGAAAC-3’
H3.3K9M Reverse Primer 5’-ATTCGCGCGTACGTATAC-3’
H3.3K27M Reverse Primer 5’-ACGCGATAACGTCGACTA-3’
H3.3G34R Reverse Primer 5’-TATCGCGTCGTACGATCG-3’
H3.3G34V Reverse Primer 5’-CGCGATTCGCGTAATACG-3’
H3.3G34W Reverse Primer 5’-GCGACGCGTAATCGA-3’
H3.3K36M Reverse Primer 5’-TAATCGACGCGTTACGC-3’

View technical datasheet for this product.  18-6201 Datasheet

 18-6201 melt curve

Melt curve: SYBR Green quantitative PCR using Input DNA from a chromatin immunoprecipitation experiment (ChIP-qPCR) where K-562 cell human chromatin was spiked-in with the SNAP-ChIP OncoStat Panel (EpiCypher Catalog No. 19-1001). PCR products of the indicated primer sets were subjected to a melting curve analysis, where the presence of a single peak indicates specific amplification of one product. Data shown is for triplicate qPCR reactions in a single ChIP experiment.
(Click image to enlarge)

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