SNAP-ChIP® K-MetStat™ H4K20 MiniPanel Primer Set

$95.00
SKU: 18-6106
Pack Size: 100 reactions
SNAP-ChIP® K-MetStat™ H4K20 MiniPanel Primer Set: The SNAP-ChIP® K-MetStat™ H4K20 MiniPanel Primer Set contains a universal forward primer pre-mixed with reverse primers that uniquely amplify DNA-barcoded nucleosomes in the SNAP-ChIP KMetStat Panel (Catalog No. 19-1001) consisting of unmodified H3.1, H4K20me1, H4K20me2, and H4K20me3. These primers can be used in SNAP-ChIP experiments where quantitative PCR (qPCR) provides an assessment of antibody performance and technical variability. The primers are compatible with SYBR™ chemistry. For TaqMan® applications, the universal SNAP-ChIP Dual Label Hydrolysis Probe can be purchased separately (Catalog No. 18-6001). The volume is sufficient to perform 100 x 10 μL qPCR reactions (see SNAP-ChIP manual).

SNAP-ChIP® K-MetStat™ H4K20 MiniPanel Primer Set Formulation: K-MetStat universal forward primer pre-mixed with individual reverse primers at 5 μM (20X) individual primer concentration in Molecular Grade H2O.

SNAP-ChIP® K-MetStat™ H4K20 MiniPanel Primer SetStorage and Stability: Stable for 2 years at -20°C from date of receipt.

SNAP-ChIP® K-MetStat™ H4K20 MiniPanel Primer Set Application Notes: For use in SNAP-ChIP-qPCR. Compatible with the SNAP-ChIP KMetStat Panel (Catalog No. 19-1001) and the SNAP-ChIP Dual Label Hydrolysis Probe (Catalog No. 18-6001).

DNA Sequence:

SNAP-ChIP K-MetStat Forward Primer 5’-CGTATCGCGCGCATAATA-3’
Unmodifed Reverse Primer 5’-CGCGTAACGACGTACC-3’
H4K20me1 Reverse Primer 5’-CGCGAACTATCGTCGATTC-3’ H4K20me2 Reverse Primer 5’-CGATACGCCGATCGATC-3’ H4K20me3 Reverse Primer 5’-CGATTCGACGGTCGC-3’

View technical datasheet for this product. 18-6106 Datasheet

18-6106 melt curve

Melt curve: SYBR Green quantitative PCR using Input DNA from a chromatin immunoprecipitation experiment (ChIP-qPCR) where K-562 cell human chromatin was spiked-in with the SNAP-ChIP K-MetStat Panel (EpiCypher Catalog No. 19-1001). PCR products of the indicated primer sets were subjected to a melting curve analysis, where the presence of a single peak indicates specific amplification of one product. Data shown is for triplicate qPCR reactions in a single ChIP experiment.
(Click image to enlarge)

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