SNAP-ChIP® K-MetStat™ H3K9 MiniPanel Primer Set: The SNAP-ChIP® K-MetStat™ H3K9 MiniPanel Primer Set contains a
universal forward primer pre-mixed with reverse primers that
uniquely amplify DNA-barcoded nucleosomes in the SNAP-ChIP KMetStat
Panel (Catalog No. 19-1001) consisting of unmodified H3.1,
H3K9me1, H3K9me2, and H3K9me3. These primers can be used in
SNAP-ChIP experiments where quantitative PCR (qPCR) provides an
assessment of antibody performance and technical variability.
The primers are compatible with SYBR™ chemistry. For TaqMan®
applications, the universal SNAP-ChIP Dual Label Hydrolysis Probe can
be purchased separately (Catalog No. 18-6001). The volume is
sufficient to perform 100 x 10 μL qPCR reactions (see SNAP-ChIP
manual).
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SNAP-ChIP® K-MetStat™ H3K9 MiniPanel Primer Set Formulation: K-MetStat universal forward primer pre-mixed with individual reverse
primers at 5 μM (20X) individual primer concentration in Molecular
Grade H2O.
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| SNAP-ChIP® K-MetStat™ H3K9 MiniPanel Primer SetStorage and Stability: Stable for 2 years at -20°C from date of receipt.
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SNAP-ChIP® K-MetStat™ H3K9 MiniPanel Primer Set Application Notes: For use in SNAP-ChIP-qPCR. Compatible with the SNAP-ChIP KMetStat
Panel (Catalog No. 19-1001) and the SNAP-ChIP Dual Label
Hydrolysis Probe (Catalog No. 18-6001).
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| DNA Sequence:
SNAP-ChIP K-MetStat Forward Primer 5’-CGTATCGCGCGCATAATA-3’
Unmodifed Reverse Primer 5’-CGCGTAACGACGTACC-3’
H3K9me1 Reverse Primer 5’-TCGCGACGGACGTA-3’
H3K9me2 Reverse Primer 5’-CGCATATCGCGTCGT-3’
H3K9me3 Reverse Primer 5’-GAACGATTCGACGATCGT-3’ |
| View technical datasheet for this product.  | |
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| Melt curve: SYBR Green quantitative PCR using Input DNA from a chromatin immunoprecipitation experiment (ChIP-qPCR) where K-562 cell human chromatin was spiked-in with the SNAP-ChIP K-MetStat Panel (EpiCypher Catalog No. 19-1001). PCR products of the indicated primer sets were subjected to a melting curve analysis, where the presence of a single peak indicates specific amplification of one product. Data shown is for triplicate qPCR reactions in a single ChIP experiment.
(Click image to enlarge) | |
