SNAP-ChIP® K-MetStat™ Full Panel Primer Set

$195.00
SKU: 18-6101
Pack Size: 100 reactions
SNAP-ChIP® K-MetStat™ Full Panel Primer Set: The SNAP-ChIP® K-MetStat™ Full Panel Primer Set contains a universal forward primer pre-mixed with reverse primers that uniquely amplify DNA-barcoded nucleosomes in the SNAP-ChIP KMetStat Panel (Catalog No. 19-1001) . These primers can be used in SNAP-ChIP experiments where quantitative PCR (qPCR) provides an assessment of antibody performance and technical variability. The primers are compatible with SYBR™ chemistry. For TaqMan® applications, the universal SNAP-ChIP Dual Label Hydrolysis Probe can be purchased separately (Catalog No. 18-6001). The volume is sufficient to perform 100 x 10 μL qPCR reactions (see SNAP-ChIP manual).
SNAP-ChIP® K-MetStat™ Full Panel Primer Set Formulation: K-MetStat universal forward primer pre-mixed with individual reverse primers at 5 μM (20X) individual primer concentration in Molecular Grade H2O.

SNAP-ChIP® K-MetStat™ Full Panel Primer SetStorage and Stability: Stable for 2 years at -20°C from date of receipt.

SNAP-ChIP® K-MetStat™ Full Panel Primer Set Application Notes: For use in SNAP-ChIP-qPCR. Compatible with the SNAP-ChIP KMetStat Panel (Catalog No. 19-1001) and the SNAP-ChIP Dual Label Hydrolysis Probe (Catalog No. 18-6001).

DNA Sequence:

SNAP-ChIP K-MetStat Forward Primer 5’-CGTATCGCGCGCATAATA-3’
Unmodifed Reverse Primer 5’-CGCGTAACGACGTACC-3’
H3K4me1 Reverse Primer 5’-CGTTAACGCGTTTCGT-3’
H3K4me2 Reverse Primer 5’-CGTACGTCGTGTCGAA-3’
H3K4me3 Reverse Primer 5’-GTTCGCGACACCGTTC-3’
H3K9me1 Reverse Primer 5’-TCGCGACGGACGTA-3’
H3K9me2 Reverse Primer 5’-CGCATATCGCGTCGT-3’
H3K9me3 Reverse Primer 5’-GAACGATTCGACGATCGT-3’
H3K27me1 Reverse Primer 5’-TACGTGTCGCGCGTA-3’
H3K27me2 Reverse Primer 5’-GAACGTTCGTCGACGAT-3’
H3K27me3 Reverse Primer 5’-GAATCGTCGACGCGTATA-3’
H3K36me1 Reverse Primer 5’-CGAAATTCGTATACGCGTCG-3’
H3K36me2 Reverse Primer 5’-GTGATATCGCGTTAACGTCG-3’
H3K36me3 Reverse Primer 5’-CGTAATGCGCGACGTTA-3’
H4K20me1 Reverse Primer 5’-CGCGAACTATCGTCGATTC-3’
H4K20me2 Reverse Primer 5’-CGATACGCCGATCGATC-3’
H4K20me3 Reverse Primer 5’-CGATTCGACGGTCGC-3’

View technical datasheet for this product. 18-6101 Datasheet

18-6101 melt curve

Melt curve: SYBR Green quantitative PCR using Input DNA from a chromatin immunoprecipitation experiment (ChIP-qPCR) where K-562 cell human chromatin was spiked-in with the SNAP-ChIP K-MetStat Panel (EpiCypher Catalog No. 19-1001). PCR products of the indicated primer sets were subjected to a melting curve analysis, where the presence of a single peak indicates specific amplification of one product. Data shown is for triplicate qPCR reactions in a single ChIP experiment.
(Click image to enlarge)

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