Recombinant SMYD2 Enzyme Description: Recombinant full-length human SMYD2 protein, (KMT3C, accession Q9NRG4), containing an N-terminal GST tag, expressed in E. coli. SMYD2 is a lysine mono-methyltransferase that has been shown to have activity on histone H3, p53 (Lys370), pRB (Lys380) and HSP90 (Lys616). The SMYD2 locus is amplified in lung carcinoma, serous ovarian carcinoma, and pancreatic ductal adenocarcinoma. SMYD2 expression is linked to a variety of cancers. Elevated levels of SMYD2 in esophageal squamous cell carcinoma patients are correlated with lower rates of survival.
Recombinant SMYD2 Enzyme Formulation: Recombinant GST-SMYD2 (1 µg/µl) in 100mM Tris pH 8.0, 10 mM glutathione and 10% glycerol.
Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Application Notes: Recombinant SMYD2, human is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 1-3 µg SMYD2 per reaction with recombinant histone H3 as a substrate is recommended.
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Enzyme Activity Data: Recombinant human SMYD2 (1 µg) was used in a methylation assay with 1 µg recombinant human histone H3 and radioactive SAM and the reaction was run on a PAGE gel. Lane 1: Coomassie stain of the gel. Lane 2: Autoradiograph. Migration of SMYD2 and H3 is indicated. (Click image to enlarge)
Protein Gel Data: Recombinant human SMYD2 (1 µg) run on a PAGE gel and stained with Coomassie blue. Migration and molecular weight of protein standards is indicated. (Click image to enlarge)