Nucleosome, Recombinant Human, H3.3S31phos dNuc, Biotinylated

SKU: 16-0389
Pack Size: 50 µg
Nucleosome, Recombinant Human, H3.3S31phos dNuc, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietarysynthetic method) contains phosphoserine at position 31. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers, is useful for nucleosome assembly, and contains a 5’ biotin-TEG group.

Nucleosome, Recombinant Human, H3.3S31phos dNuc Biotinylated Formulation: Nucleosome, Recombinant Human, H3.3S31phos (27.4 μg protein weight, 50 μg DNA+protein) in μl 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 199,867 Da.

Nucleosome, Recombinant Human, H3.3S31phos dNuc, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Nucleosome, Recombinant Human, H3.3S31phos dNuc, Biotinylated Application Notes: Nucleosome, Recombinant Human, H3.3S31phos dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3.3S31phos dNucs from EpiCypher does not contain free DNA which could alter assayed activities.

Armache et al. (2020). Nature 583(7818):852-857 PMID: 32699416
Lowary PT and J Widom (1998). J Mol Biol 276: 19-42.
Luger K et al. (1999). Methods Mol Biol 119: 1-16.

View technical datasheet for this product. 16-0389 Datasheet
16-0389 Western Blot
Western Blot Data:Western Analysis of Nucleosome, Recombinant Human, H3.3S31phos. Top Panel: Unmodified H3 (Lane 1) and H3.3S31phos containing nucleosomes (Lane 2) were probed with an anti-H3.3S31phos antibody and analyzed via ECL readout. Only the H3.3S31phos sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H3.3S31phos nucleosomes (Lane 2).
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16-0389 MS Data
Mass Spec Data:H3.3S31phos protein analyzed by high resolution mass spectrometry. Expected mass = 15,276,7 Da. Determined mass = 15,276.6 Da.
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16-0389 Protein Gel Data
Protein Gel Data: Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H3.3S31phos (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3.3S31phos, and H4) are indicated.
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16-0389 DNA Gel Data
DNA Gel Data: Nucleosome, Recombinant Human, H3.3S31phos run on a native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (100 ng). Lane 2: Intact nucleosomes (400 ng).
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