Histone H3 K27Me2 Peptide, Biotinylated

SKU: 12-0018
Pack Size: 50 μg
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Histone H3 K27Me2 Peptide Description: A synthetic peptide derived from human histone H3.3, amino acids 15-34, dimethylated at lysine 27. The alpha-amino group on the N-terminus is acetylated, with a PEG spacer containing a biotin moiety at the C-terminus.

Histone H3 K27Me2 Peptide Formulation:
50 µg of lyophilized powder.

Histone H3 K27Me2 Peptide Sequence:

Molec. Weight: 2495 Da.

Purity: >80% as judged by HPLC and mass spectrometry.

Storage and Stability: Lyophilized peptides are stable for 2 years at -20°C from date of shipment. Peptides in solution are stable for 6 months at -80°C. Spin vial briefly in microfuge and reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot peptide to avoid repeated freezing and thawing.

Quality Control: Peptide purified by reversed-phase HPLC and analyzed using MALD-TOF mass spectrometry and analytical RP-HPLC.
12-0018 Mass Spec
Mass Spec Data: MALDI-TOF mass spectrometry was used to analyze histone H3 K27Me2 peptide for identity and homogeneity.

12-0018 HPLC
HPLC Data: Reversed-phase analytical HPLC chromatogram of purified histone H3 K27Me2 peptide.

References: Fuchs SM et al (2011). Curr Biol 21: 53-58.
View technical datasheet for this product. 12-0018 Datasheet
Histone H3 K27Me2 Peptide, Biotinylated, for use in peptide pull down or binding assays.
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