Histone H4K5,8,12but Peptide, Biotinylated

SKU: 12-0227
Pack Size: 50 μg
Histone H4K5,8,12but Peptide, Biotinylated Description: A synthetic peptide derived from human histone H4, amino acids 1-23, butyrylated at lysines 5, 8, and 12. The alpha-amino group on the N-terminus is acetylated, with a C-terminal PEG spacer containing a biotin moiety.

Histone H4K5,8,12but Peptide, Biotinylated, Formulation:
50 µg of lyophilized powder.

Histone H4K5,8,12but Peptide, Biotinylated Sequence:
Ac-SGRGK(but)GGK(but)GLGK(but)GGAKRHRKVLR-Peg (Biot)

Molec. Weight: 3041.7 Da

Purity: >90% as judged by HPLC and mass spectrometry.

Storage and Stability: Lyophilized peptides are stable for 2 years at -20°C from date of shipment. Peptides in solution are stable for 6 months at -80°C. Spin vial briefly in microfuge and reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot peptide to avoid repeated freezing and thawing.

Quality Control: Peptide purified by reversed-phase HPLC and analyzed using MALDI-TOF mass spectrometry and analytical RP-HPLC.
12-0227 Mass Spec Data
Mass Spec Data: MALDI-TOF mass spectrometry was used to analyze Histone H4K5,8,12but Peptide, for identity and homogeneity.

12-0227 HPLC Data
HPLC Data: Reversed phase analytical HPLC chromatogram of purified Histone H4K5,8,12but Peptide.

References: Fuchs SM et al (2011). Curr Biol 21: 53-58.
View technical datasheet for this product. 12-0227 Datasheet
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