SKU: 18-1401
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=748","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Epigenetics Reagents and Assays","Epigenetics Reagents and Assays/CUTANA™ ChIC / CUT&RUN Assays"],"custom_fields":[{"id":"510","name":"Pack size","value":"100 ng"}],"customizations":[],"description":"<html xmlns=\"http://www.w3.org/1999/xhtml\">\n<head>\n<meta http-equiv=\"Content-Type\" content=\"text/html; charset=UTF-8\" />\n<title>Untitled Document</title>\n</head>\n\n<body>\n\n<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 12pt;\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong>CUTANA <i>E. coli</i> Spike-in DNA Description</strong></span></span>:</strong><span style=\"font-family: Arial; font-size: 12pt;\"> Fragmented DNA derived from <i>Escherichia coli</i> (<i>E. coli</i>) can be used as a spike-in control for experimental normalization in Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUTANA <i> E. coli</i> Spike-in DNA contains sufficient material for 100-200 CUT&RUN samples (high and low abundance targets, respectively).</span><br /><br> </td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong>CUTANA<sup>™</sup> <i>E. coli</i> Spike-in DNA Description</strong></span></span> Formulation:</strong> 100 ng lyophilized DNA. *NOTE: May not be visible.<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong>CUTANA <i>E. coli</i> Spike-in DNA Description Storage and Stability:</strong> Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.</span></span><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><br /> <br /> </span></span></p> </td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong><strong>CUTANA <i>E. coli</i> Spike-in DNA Description</strong> Application Notes:</strong><br> Prior to opening, pellet DNA by quick spin in a benchtop microfuge. Reconstitute in 200 µL DNase free water (0.5 ng/µL). Vortex tube on all sides to ensure complete resuspension.<br><br>\n\n\n\n<strong>Use in CUT&RUN:</strong><br>\n\n<strong>1.</strong> Use in combination with CUTANA<sup>™</sup> pAG-MNase for ChIC/CUT&RUN (EpiCypher 15-1016), which has very low levels of <i>E. coli</i> DNA.<br>\n\n\n\n<strong>2.</strong> Add 1-2 µL* (0.5-1 ng) <i>E. coli</i> Spike-in DNA directly to the Stop Buffer, which quenches calcium-mediated pAG-MNase DNA digestion (see protocol: <a href=\"https://www.epicypher.com/resources/protocols/\">www.epicypher.com/resources/protocols/</a>). Gently vortex to mix, and add to CUT&RUN samples. <i>*NOTE: Based on target abundance and antibody efficiency, the amount of <i>E. coli</i> DNA may need to be adjusted. Aim for spike-in DNA to comprise ~1% (0.2-5%) of total sequencing reads (see Table, right).</i><br>\n\n\n\n<strong>3.</strong> After sequencing, align reads to the reference genome from the experimental samples (<i>e.g.</i> human) as well as to the <i>E. coli</i> genome.<br>\n\n\n<strong>4.</strong> Normalize data by following recommendations described in the EpiCypher CUTANA CUT&RUN Protocol: <a href=\"https://www.epicypher.com/resources/protocols/\">www.epicypher.com/resources/protocols/</a>.<br>\n\n<br /> </span></span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br /> <span style=\"font-size: 10pt;\"> </span></p> </td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/18-1401.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"18-1401 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> <p></p> </td> </tr> </tbody> </table> </td> \n\n<td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\" valign=\"top\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/18-1401-DNA.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/18-1401-DNA.jpg\" alt=\"18-1401 Gel Data\" width=\"241\" height=\"400\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>DNA Gel Data:</strong> Lane 1: gDNA extracted from JM101 <i>E. coli</i> cells (500 ng) and Lane 2: Digested and purified CUTANA <i>E. coli</i> Spike-in DNA (500 ng) resolved via 2% E-Gel™ EX Agarose Gel. Migration positions of DNA molecular weight markers are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td> </tr> </tbody> </table>\n <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\" valign=\"top\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/18-1401-Seq.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/18-1401-Seq.jpg\" alt=\"18-1401 Sequencing Data\" width=\"241\" height=\"400\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>CUT&RUN Sequencing Data:</strong> CUTANA <i>E. coli</i> Spike-in DNA (0.5 and 1.0 ng) was added to CUT&RUN samples using 500,000 K562 cells enriched for a low abundance target (H3K4me3, EpiCypher Catalog No. 13-0041), a high abundance target (H3K27me3, EpiCypher Catalog No. 13-0030) and IgG negative control (EpiCypher Catalog No. 13-0042). Total numbers of paired-end sequencing reads, reads aligned to <i>E. coli</i>, and percentage of total sequencing reads aligned to <i>E. coli</i> spike-in DNA are shown. Green boxes highlight the recommended <i>E. coli</i> DNA spike-in amounts for each target.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td> </tr> </tbody> </table> </td>\n\t\n\t\n\t</td>\n\n\n\n</tr> </tbody> </table> </div>","detail_messages":"","gift_wrapping_available":false,"id":"748","images":[{"alt":"CUTANA™ E. coli Spike-in DNA","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/748/730/DNA_Vector_Draw_Final-w-border-01__76823.1592491202.png?c=2"}],"main_image":{"alt":"CUTANA™ E. coli Spike-in DNA","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/748/730/DNA_Vector_Draw_Final-w-border-01__76823.1592491202.png?c=2"},"max_purchase_quantity":0,"meta_description":"CUTANA™ E. coli Spike-in DNA","meta_keywords":"CUTANA™ CUT&RUN E. coli Spike-in DNA, E. coli spike-in DNA, spike-in DNA, CUT&RUN, cut and run, cleavage under targets and released using nuclease, ChIC, chromatin immunocleavage, 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Pack size: 100 ng
