SETD6 Antibody

SKU: 13-0003
Pack Size: 100 µg
Type:  Polyclonal Host:  Rabbit
Mol Wgt.:  53 kDa Appl.:  IF, IP, WB
Format:  Aff. Pur. IgG Reactivity:  Human

SETD6 antibody Description:

Affinty purified antibody to SETD6. SETD6 is a SET domain-containing lysine methyl-transferase that monomethylates the RelA subunit of NF-kB at lysine 310. Methylation of RelA inactivates it, attenuating RelA-dependant transcriptional pathways, including inflammatory responses in primary immune cells. SETD6 is downregulated in a diseases of hyper inflammation. SETD6 has also been reported to have other nuclear substrates.

SETD6 antibody Immunogen:

Recombinant protein corresponding to full-length GST-tagged human SETD6 (NP_001153777).

SETD6 antibody Formulation:

200 µl immunoaffinity purified rabbit IgG at 0.5 mg/ml in PBS, pH7.5, 30% glycerol and 0.035% sodium azide.

SETD6 Storage and Stability:

Stable for 2 years at -20°C from date of receipt.

SETD6 Application Notes:

This SETD6 antibody can be used in immunofluorescence (IF, 1-2 µg/ml), immunoprecipitation (IP, 2-5 µg per IP) and Western blotting (0.2 - 1 µg/ml).

SETD6 References:

Levy D et al (2011). Nat Immunol 12: 29-36. Link
View technical datasheet for this product. 13-0003 Datasheet
13-0003 IF
Immunofluorescence Data: IF performed on U2OS cells with SETD6 antibody (Green, left) and counterstained with DAPI (Blue, right). Bottom left: U2OS cells transfected with anti-SETD6 siRNA.
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