SETD2 Antibody

SKU: 13-0025
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Type:  Polyclonal Host:  Rabbit
Mol Wgt.:  250 kDa Appl.:  WB
Format:  Aff. Pur. IgG Reactivity:  H

SETD2 Antibody Description: SETD2 (SET2, HYPB, HIF1, KMT3A, accession Q9BYW2, amino acids 1345-1711) is a SET-domain containing histone methyltransferase, catalyzing the trimethylation of histone H3 at lysine 36. SETD2 is a candidate tumor suppressor protein and mutated in clear cell renal cell carcinoma.

SETD2 Antibody Immunogen: Recombinant protein corresponding to amino acids amino acids 1345-1711 of human SETD2.

SETD2 Antibody Formulation: Immunoaffinity purified rabbit IgG in PBS (pH 7.4) with 0.02% sodium azide and 1% BSA.

Storage and Stability: Stable for 2 years at -20°C from date of receipt.

Application Notes / Recommended dilutions:
   WB 1:500 - 1:2,000


View technical datasheet for this product. 13-0025 Datasheet
View additional specificity data for this product. 13-0025 Array Spec Data

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-0025 Western Blot

Western Blot Data: Western blot using SETD2 Antibody (1:500 dilution). Lane 1: Whole cell extract (50,000 whole cell equivalents) from HKC cells transformed with a random shRNA construct. Lane 2: Whole cell extract (50,000 whole cell equivalents) from HKC cells transformed with construct stably expressing an shRNA targeting SETD2.
(Click image to enlarge)

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