Histone H3 K9Me1 Antibody

SKU: 13-0014
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Type:  Polyclonal Host:  Rabbit
Mol Wgt.:  15 kDa Appl.:  IF, IHC, IP, WB
Format:  Aff. Pur. IgG Reactivity:  H, Sc, WR
Histone H3 K9Me1 Antibody Description: Rabbit polyclonal antibody recognizing the histone H3 monomethylated at lysine 9. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Methylation of lysine 9 is associated with transcriptional repression and the formation of heterochromatin through the recruitment of K9 methyl-binding proteins that facilitate the compaction of chromatin.

Histone H3 K9Me1 Antibody Immunogen: Synthetic peptide corresponding to the amino terminus of human histone H3 monomethylated at lysine 9.

Histone H3 K9Me1 Antibody Formulation: Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.

Histone H3 K9Me1 Antibody Quality Control: This antibody has been validated for use in IF and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
  • Is specific for H3 K9Me1
  • Has no cross-reactivity to related modifications
  • Is unaffected by nearby modifications

Histone H3 K9Me1 Antibody Storage and Stability: Stable for 2 years at -20°C from date of receipt.

Histone H3 K9Me1 Antibody Application Notes Recommended dilutions:
   IF/IHC  1:50 - 1:200
   IP  2 - 5 µg per IP
   WB  1:500 - 1:2000

Histone H3 K9Me1 Antibody References:
View technical datasheet for this product. 13-0014 Datasheet
View additional specificity data for this product. 13-0014 Array Spec Data

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-0014 Western Blot

Western Blot Data: HeLa cell extract (HeLa) and recombinant histone H3 (H3 protein) were blotted onto PVDF and probed with Histone H3 K9Me1 Antibody.
(Click image to enlarge)

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