GST Epitope Tag Antibody

SKU: 13-0022
Pack Size: 100 μg
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Type:  Polyclonal Host:  Rabbit
Mol Wgt.:  N/A Appl.:  IF, WB
Format:  Serum Reactivity:  N/A

GST Epitope Tag Antibody Description:

Glutathione S-transferase (GST) is a 26 kDa protein from Schistosoma japonica and is used as an epitope tag for improving the expression and solubility of GST fusion proteins expressed in E coli. This antibody is useful for detection of GST-tagged proteins on the EpiTitan Histone Peptide array and for detection of GST-tagged fusion proteins by Western Blot, including proteins bound to specific peptides in pull-down experiments.

GST Epitope Tag Antibody Immunogen:

Purified recombinant GST from Schistosoma japonica.

GST Epitope Tag Antibody Formulation:

Rabbit serum with 30% glycerol and 0.035% sodium azide.

GST Epitope Tag Antibody Storage and Stability:

Stable for 2 years at -20°C from date of receipt. 

GST Epitope Tag Antibody Application Notes

Recommended dilutions:
   IF  1:500 - 1:2,000
   WB  1:1,000 - 1:5,000

GST Epitope Tag Antibody References:

Hacker KE et al (2016). J Biol Chem DOI: 10.1074/jbc.M116.739375 LINK
View technical datasheet for this product. 13-0022 Datasheet
13-0022 Epititan Image

Immunofluorescence Data: GST Epitope Tag Antibody (1:1,000 dilution) used to label a GST-tagged fusion protein bound to specific peptides on the EpiTitan Histone Peptide Array (Catalog No. 11-2001). The GST antibody was then detected using an AlexaFluor 647-conjugated secondary antibody (red).
(Click image to enlarge)

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

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