Histone H3 R8Me1 Antibody

SKU: 13-0017
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Type:  Polyclonal Host:  Rabbit
Mol Wgt.:  15 kDa Appl.:  ChIP, IF, IHC, WB
Format:  Aff. Pur IgG Reactivity:  H, WR

Histone H3 R8Me1 Antibody Description:

Rabbit polyclonal antibody recognizing the histone H3 monomethylated at arginine 8. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Monomethylation of arginine 8 is catalyzed by PRMT2 and PRMT5.

Histone H3 R8Me1 Antibody Immunogen:

Synthetic peptide corresponding to a region of human histone H3 monomethylated at arginine 8.

Histone H3 R8Me1 Antibody Formulation:

Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.

Histone H3 R8Me1 Antibody Quality Control:

This antibody has been validated for use in IF, IHC, IP and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
  • Is specific for H3 R8Me1
  • Has no cross-reactivity to related modifications
  • Is unaffected by nearby modifications

Histone H3 R8Me1 Antibody Storage and Stability:

Stable for 2 years at -20°C from date of receipt. 

Histone H3 R8Me1 Antibody Application Notes

Recommended dilutions:
   ChIP  2 - 5 µg per IP
   IF/IHC  1:50 - 1:200

   WB  1:500 - 1:2,000

Histone H3 R8Me1 Antibody References:

View technical datasheet for this product. 13-0017 Datasheet
View additional specificity data for this product. 13-0017 Datasheet

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-0017 Epititan Image

Peptide Array Specificity Data: EpiTitan™ Histone Peptide Array used to analyze histone modification specificity of Histone H3 R8Me1 Antibody (Arg8 monomethyl) catalog number 13-0017. Antibody signal appears in red, while the spotting tracer appears in green.
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13-0017 Epititan Specificity

Specificity Data-Array: EpiTitan™ Histone Peptide arrays used to analyze the histone modification specificity of Histone H3 R8Me1 Antibody. Data is shown for the highest reacting singly-modified peptides. For more specificity data, see Additional Peptide Array Specificity Data.
(Click image to enlarge)

13-0017 Dot Blot

Specificity Data-Dot Blot: Peptide dot blot used to confirm the specificity of Histone H3 R8Me1 Antibody. Methylated peptides corresponding to the immunogen and related sites were spotted onto PVDF and probed with the antibody.
(Click image to enlarge)

13-0017 IF

Immunofluorescence Data: HEK293T cells stained with Histone H3R8Me1 Antibody (red) and counterstained with DAPI (blue).
(Click image to enlarge)

13-0017 Western Blot

Western Blot Data: HeLa cell extract (HeLa) and recombinant histone H3 (H3 protein) were blotted onto PVDF and probed with Histone H3 R8Me1 Antibody.
(Click image to enlarge)

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