Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag – 50 reactions
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- 250 Reactions
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- Type: Mixed Monoclonal*
- Host: Goat
- Applications: CUT&Tag
- Reactivity: Anti-Mouse
- Format: Protein G affinity-purified
- Target Size: N/A
Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag is affinity-purified and specific for mouse immunoglobulins. Minimal cross reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope [Kaya-Okur et al., 2019]. For best results, use with CUTANA™ pAG-Tn5 (EpiCypher 15-1017 / 15-1117). For detailed instructions for use, see EpiCypher’s CUTANA™ CUT&Tag protocol.
*Mixed Monoclonal: a pool of multiple recombinant monoclonal antibodies.
Figure 1: CUT&Tag data
CUT&Tag was performed as described in Figure 2. Gene browser shots generated using the Integrative Genomics Viewer (IGV, Broad Institute) show a representative 474 kb window centered at the LAMC3 gene. The genomic distribution pattern was consistent with that expected for H3K27me3.
Figure 2: CUT&Tag methods
CUT&Tag was performed on 100k K562 nuclei with 0.5 µg of either IgG (Invitrogen 10400C) or H3K27me3 (Abcam ab6002) antibodies, followed by 0.5 µg of anti-mouse secondary antibody for CUTANA™ CUT&Tag. The EpiCypher Direct-to-PCR CUT&Tag protocol was used. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 2.0 million reads (IgG) and 5.8 million reads (H3K27me3). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.