SKU: 16-0337
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k18cr-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"598","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Nucleosome, Recombinant Human, H3K18cr dNuc, Biotinylated","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/598/547/Recombinant_nucleosome__69426.1538135202.png?c=2","alt":"Nucleosome, Recombinant Human, H3K18cr dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=598","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"854","name":"Pack Size","value":"50 μg"},{"id":"855","name":"Internal Comment","value":"extra in bottom right of Psylocke"}],"sku":"16-0337","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K18cr dNuc,\nBiotinylated Description:</b> Mononucleosomes assembled from recombinant human\nhistones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2BO60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains crotonyl-lysine at position 18. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by\nLowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group. * H3K18cr has a Cys to Ala substitution at position 110.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K18cr</b></span></span> dNuc Biotinylated Formulation:</b> Nucleosome, Recombinant Human, H3K18cr (27.3 μg protein weight, 50.0 μg DNA+protein) in 10mM Tris HCl, pH 7.5, 25mM NaCl, 1mM EDTA, 2mM DTT, 20% glycerol. MW = 199,997.8 Da.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K18cr dNuc Biotinylated Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For\nbest results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K18cr dNuc Biotinylated Application Notes:</b> Nucleosome, Recombinant Human, H3K18cr are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K18cr from EpiCypher does not contain free DNA which could alter assayed activities.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0337.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0337 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0337-WB-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0337-WB-data.jpg\" alt=\"16-0337 Western Blot\" width=\"125\" height=\"325\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong>Western Analysis of Nucleosome,\nRecombinant Human, H3K18cr. Top Panel: Unmodified H3 (Lane 1) and H3K18cr containing nucleosomes (Lane 2) were probed with an anti-H3K18cr antibody and analyzed via ECL readout. Only the H3K18cr sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified\nnucleosomes (Lane 1) and H3K18cr nucleosomes (Lane 2).<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0337-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0337-MS-data.jpg\" alt=\"16-0337 MS Data\" width=\"125\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong>Synthetic H3K18cr protein analyzed by high resolution mass spectrometry. Expected mass = 15,291.8 Da. Determined mass = 15,292.5 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0337-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0337-protein-gel.jpg\" alt=\"16-0337 Protein Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in\nNucleosome, Recombinant Human, H3K18cr (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K18cr and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0337-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0337-DNA-gel.jpg\" alt=\"16-0337 DNA Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H3K18cr via native PAGE and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (100 ng). <strong>Lane 2:</strong> Intact H3K18cr containting nucleosomes (400 ng).<br /> <strong>(Click image to enlarge) 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Pack Size: 50 μg
