Histone H3K4me3 Antibody, SNAP-ChIP Certified

SKU: 13-0028
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Type:  Monoclonal Host:  Rabbit
Mol Wgt.:  15 kDa Appl.:  ChIP, ChIP-Seq, ELISA
Format:  Aff. Pur. IgG Reactivity: H, M, WR
Histone H3K4me3 Antibody - SNAP ChIP Certified Description: Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). This antibody reacts to H3K4me3 and no cross reactivity with H3K4me1 or H3K4me2, or other lysine methylations in the EpiCypher SNAP-ChIP K-MetStat panel, is detected.
Histone H3K4me3 Antibody - SNAP ChIP Certified Immunogen: A synthetic peptide corresponding to histone H3 trimethylated at lysine 4.

Histone H3K4me3 Antibody - SNAP ChIP Certified Formulation: Protein A affinity-purified antibody (1 mg/mL) in PBS, with 0.09% sodium azide, 1% BSA, and 50% glycerol.

Histone H3K4me3 Antibody - SNAP ChIP Certified Storage and Stability: Stable for 1 years at -20°C from date of receipt.

Histone H3K4me3 Antibody - SNAP ChIP Certified Application Notes Recommended dilutions:
ChIP 2 - 5 μg per 1x106 cells ELISA 1 - 10 μg/mL
Histone H3K4me3 Antibody - SNAP ChIP Certified References:

View technical datasheet for this product. 13-0028 Datasheet

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-0028 SNAP-ChIP Data

SNAP-ChIP qPCR Data: Histone H3K4me3 antibody (3 μg) was tested in a native ChIP experiment with chromatin from HEK-293 cells (~1x106 cells). Specificity (left Y-axis) was determined by qPCR for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel(EpiCypher Catalog No. 19-1001, X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input. The antibody exhibited good specificity for the target (<13% cross-reactivity outside H3K4me3) and high efficiency (18.7%). Error bars represent mean ± SEM from two barcoded nucleosome replicates in two ChIP experiments.
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13-0028 ELISA

ELISA Data: Designer Nucleosomes (dNucs) containing the indicated H3K4 modification were used in an ELISA assay with Histone H3K4me3 Antibody (10 μg/mL).
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