Histone H3K4me2 Antibody, SNAP-ChIP Certified

SKU: 13-0013
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Type:  Polyclonal Host:  Rabbit
Mol Wgt.:  15 kDa Appl.:  IF, WB
Format:  Aff. Pur. IgG Reactivity: ChIP, ChIP-seq, IF, IHC, IP, WB
Histone H3K4me2 Antibody Description: Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Methylation of lysine 4 of histone H3 (by SET1, ASH1 or the MLL family of methyltransferases) is associated with transcriptional activation, and also frequently found in the context of H3 acetylation at multiple residues.
Histone H3K4me2 Antibody Immunogen: Synthetic peptide derived from human histone H3 monomethylated at lysine 27.

Histone H3K4me2 Antibody Formulation: Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.

Histone H3K4me2 Antibody Quality Control: This antibody has been validated for use in IF, IHC and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
  • Is specific for H3K4me2
  • Has no cross-reactivity to related modifications
  • Is unaffected by nearby modifications

Histone H3K4me2 Antibody Storage and Stability: Stable for 2 years at -20°C from date of receipt.

Histone H3K4me2 Antibody Application Notes Recommended dilutions:
   ChIP  2-5 µg per 1x10^6 cells
   IF / IHC  1:50 - 1:200
   IP  2-5 µg per IP
   WB  1:500 - 1:2,000

Histone H3K4me2 Antibody References:

View technical datasheet for this product. 13-0013 Datasheet
View additional specificity data this product. 13-0013 Array Spec Data

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-0013 SNAP-ChIP Data

SNAP-ChIP qPCR Data: Histone H3K4me2 antibody (1:60 dilution) was tested in a native ChIP experiment with chromatin from HEK-293 cells (~1x10^6 cells). Specificity (left Y-axis) was determined by qPCR for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (EpiCypher Catalog No. 19-1001, X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me2 nucleosome target immunoprecipitated relative to Input. The antibody exhibited good specificity for the target (<10% cross-reactivity outside H3K4me2) and high efficiency (18.1%). Error bars represent mean ± SEM from two barcoded nucleosome replicates in a single ChIP experiment.
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13-0013 Epititan Specificity

Specificity Data-Array: EpiTitan Histone Peptide arrays used to analyze the histone modification specificity of Histone H3K4me2 Antibody. Data is shown for the highest reacting singly-modified peptides. For more specificity data, see Additional Peptide Array Specificity Data.
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13-0013 Dot Blot

Specificity Data-Dot Blot: Peptide dot blot used to confirm the specificity of Histone H3K4me2 Antibody. Methylated peptides corresponding to the immunogen and related sites were spotted onto PVDF and probed with the antibody.
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13-0013 IF

Immunofluorescence Data: HEK293T cells stained with Histone H3K4me2 Antibody (red) and counterstained with DAPI (blue).
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13-0013 Western Blot

Western Blot Data: HeLa cell extract (HeLa) and recombinant histone H3 (H3 protein) were blotted onto PVDF and probed with Histone H3K4me2 Antibody.
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