Histone H3K4ac Antibody - SNAP-ChIP® Certified

$405.00
SKU: 13-0034
Pack Size: 100 μg
Type:  Monoclonal Host:  Rabbit
Mol Wgt.:  15 kDa Appl.:  ChIP, ChIP-Seq, WB, ICC, L
Format:  Aff. Pur. IgG Reactivity: H, M, WR
Histone H3K4ac Antibody, SNAP-ChIP Certified Description: This antibody meets EpiCypher’s “SNAP-ChIP® Certified” criteria for specificity and efficient target enrichment in a ChIP experiment (<20% cross-reactivity across the panel, >5% recovery of target input). Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). This antibody reacts to H3K4ac when present alone as well as in combination with nearby acetylations (tetraAc-H3) and no cross reactivity with other lysine acylations in the EpiCypher SNAP-ChIP K-AcylStat panel, is detected.
 
Histone H3K4ac Antibody, SNAP-ChIP Certified Certified Immunogen: A synthetic peptide corresponding to histone H3 acetylated at lysine 4.

Histone H3K4ac Antibody, SNAP-ChIP Certified Certified Formulation: Protein A affinity-purified antibody (1 mg/mL) in PBS, with 0.09% sodium azide, 1% BSA, and 50% glycerol.

Histone H3K4ac Antibody, SNAP-ChIP Certified Certified Storage and Stability: Stable for 1 years at -20°C from date of receipt.

Histone H3K4ac Antibody, SNAP-ChIP Certified Certified Application Notes Recommended dilutions:
  
ChIP: 2 - 5 μg per 1x10^6 cells WB: 1 - 2 μg/mL ICC: 0.05 - 2 μg/mL L: 0.25 - 4 μg/mL

Histone H3K4ac Antibody, SNAP-ChIP Certified Certified References:

Grzybowski et al (2015) Mol Cell 58:886

Shah et al (2018) Mol Cell 72:162

View technical datasheet for this product. 13-0034 Datasheet

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; ICC-Immunocytochemistry; L-Luminex; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-0034 SNAP-ChIP Data

SNAP-ChIP qPCR Data: Histone H3K4ac antibody (5 μg) was tested in a native ChIP experiment using chromatin from K-562 cells (10 μg) with the SNAP-ChIP K-AcylStat Panel (EpiCypher Catalog No. 19-3001) spiked-in prior to micrococcal nuclease digestion. Specificity (left Y-axis)was determined by qPCR for the DNA barcodes corresponding to modified nucleosomes in the SNAP-ChIP panel (x-axis). Black bar represents antibody efficiency (right y-axis; log scale) and indicates percentage of the target immunoprecipitated relative to input. tetraAc-H3 is K4,K9,K14,K18ac; tetraAc-H4 is K5,K8,K12,K16ac; tetraAc-H2A is K5,K8,K13,K15ac
(Click image to enlarge)

13-0034 Luminex Data

Luminex Data: Histone H3K4ac antibody was assessed using a Luminex® based approach employing dCypher® Nucleosome K-AcylStat Panel (EpiCypher Catalog No. 16-9003). The panel comprises biotinylated designer nucleosomes (X-Axis) individually coupled to uniquely identifiable Luminex Magplex® beads. Antibody binding to nucleosomes was tested in multiplex (24-plex) at a 1:1000 dilution, and detected with second layer anti-IgG*PE. Data was generated using a Luminex FlexMAP3D®. Data normalized to relevant on-target (H3K4ac; set to 100) is shown. tetraAc-H3 is K4,K9,K14,K18ac; tetraAc-H4 is K5,K8,K12,K16ac; tetraAc-H2A is K5,K8,K13,K15ac
(Click image to enlarge)

13-0034 Western Blot data

Western Blot Data: Western blot of acid extracts from HeLa cells untreated ( - ) or treated with sodium butyrate ( + ), using EpiCypher Catalog No. 13-0034 at 0.5 µg/mL.
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13-0034 ICC

Immunocytochemistry Data: Immunocytochemistry of HeLa cells treated with sodium butyrate, using H3K4ac antibody, EpiCypher Catalog No. 13-0034 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
(Click image to enlarge)

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