Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Acetylation of H3 lysine 9 (by GCN5 and PCAF in mammals) is associated with transcriptional activation.
Histone H3 K9Ac Antibody Immunogen:
Synthetic peptide corresponding to the amino terminus of human histone H3 acetylated at lysine 9.
Histone H3 K9Ac Antibody Formulation:
Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Histone H3 K9Ac Antibody Quality Control:
This antibody has been validated for use in ChIP, IF and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
Is specific for H3 K9Ac
Has no cross-reactivity to related modifications
Is unaffected by nearby modifications
Histone H3 K9Ac Antibody Storage and Stability:
Stable for 2 years at -20°C from date of receipt.
Histone H3 K9Ac Antibody Application Notes:
Recommended dilutions: ChIP 2 - 5 µg per ChIP IF/IHC 1:50 - 1:200 IP 2 - 5 µg per IP WB 1:500 - 1:2000
Histone H3 K9Ac Antibody References:
View technical datasheet for this product. View additional specificity data for this product.
Peptide Array Specificity Data: EpiTitan™ Histone Peptide Array used to analyze histone modification specificity of Histone H3 K9Ac Antibody (Lys9 Acetyl) catalog number 13-0020. Antibody signal appears in red, while the spotting tracer appears in green. (Click image to enlarge)
Chromatin IP Data: Chromatin IP (ChIP) performed on HeLa cell chromatin using Histone H3 K9Ac Antibody (black bars) or rabbit IgG (grey bars) as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primers specific for the gene indicated. Data are presented as ratios relative to input DNA in ChIP chromatin. (Click image to enlarge)
Specificity Data-Array: EpiTitan™ Histone Peptide arrays used to analyze the histone modification specificity of Histone H3 K9Ac Antibody. Data is shown for the highest reacting singly-modified peptides. For more specificity data, see Additional Peptide Array Specificity Data. (Click image to enlarge)
Immunofluorescence Data: HeLa cells stained with Histone H3 K9Ac Antibody (green) and counterstained with DAPI (blue). (Click image to enlarge)
Western Blot Data: BT-474 cell extract (BT-474), HEK293T cell extract (293T) and recombinant histone H3 (H3 protein) were blotted onto PVDF and probed with Histone H3 K9Ac Antibody. (Click image to enlarge)