Histone H3.3 Expression Vector pEPI-H3.3K27M Description: A mammalian vector encoding the expression of human histone H3.3 (accession P84243, molecular weight of 15 kDa) with a mutation substituting methionine for lysine at position 27, driven by the cytomegalovirus (CMV) promoter, containing a C-terminal DDDDK epitope tag. The vector contains an ampicillin resistance gene for bacterial selection and a neomycin gene for mammalian selection.
Histone H3.3 Expression Vector pEPI-H3.3K27M Storage and Stability: Stable for 2 years at -20°C from date of receipt.
Histone H3.3 Expression Vector pEPI-H3.3K27M Histone H3.3 Expression Vector pEPI-H3.3K27M can be used for the expression of DDDDK-tagged human histone H3.3 (K27->M) in mammalian cell culture cells.
References: Lewis PW et al (2013). Science 340: 857-861. Link
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pEPI-H3.3K27M Western Blot Data: Western blot from HEK293 cells transfected with either the pEPI empty vector (lane 1), pEPI-H3.3K27M (lane 2), pEPI-H3.3K27M (lane 3), or pEPI-H3.3K27M (lane 4). Top Panel: Western blot using anti-H3 C-terminal antibody (Catalog No. 13-0001). Bottom Panel: Western blot using anti-DDDDK epitope tag antibody. (Click image to enlarge)