| Type: Histone |
Host: E. coli |
| Mol Wgt.: 54 kDa |
Epitope Tag: None |
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Histone CENP-A/H4 Tetramers, Recombinant Human Description: CENP-A/ Histone H4 Tetramers made from recombinant histones expressed in E. coli (Centromere protein A, CENPA, CenH3; accession numbers: CENP-A: P49450; H4: P62805). CENP-A and H4 were expressed and purified individually by FPLC, then assembled into tetramers that were further purified using gel filtration chromatography. CENP-A is a specialized variant of H3 and component of modified nucleosomes found at the core centromere.
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Histone CENP-A/H4 Tetramers, Recombinant Human Formulation: 50 µg of CENP-A/H4 Tetramer in 40mM sodium phosphate buffer pH 7.0, 1M NaCl, 7mM β-ME, 50% glycerol.
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Histone CENP-A/H4 Tetramers, Recombinant Human Storage and Stability: Lyophilized proteins are stable for 2 years at -20°C from date of shipment. Reconstitute with sterile distilled water and aliquot to avoid repeated freezing and thawing. Stable for 6 months at -80°C after reconstitution.
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Histone CENP-A/H4 Tetramers, Recombinant Human Application Notes: Recombinant histone CENP-A/H4 Tetramers are suitable for enzyme assays and nucleosome reconstitution.
N.B DO NOT FREEZE. DO NOT STORE AT -80°C
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References:
Sekulic N et al (2010). Nature 476: 347-351. Link
Falk SJ et al (2015). Science 348: 699-703. Link
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View technical datasheet for this product.  |
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QC Protein Data: Histone CENP-A/H4 Tetramers, Recombinant Human, (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.
(Click image to enlarge)
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